Dragi članovi društva,
Otvorena je nova postdoc pozicija na Allen institutu u Vašingtonu
(Washington DC),na Odeljenju za molekularnu genetiku pod rukovodstvom dr
Bosiljke Tasić. Potrebno je da kandidat ima završene doktorske studije u
oblasti neurobiologije, genetike, biohemije (ili sličnim granama), kao i
da ima iskustva u tehnikama molekularnog kloniranja i da poznaje rad u
softverima Geneious i Vector NTI. S obzirom da rad podrazumeva
razvijanje transgenih linija miševa i karakterizacije novih sojeva,
poželjno je da kandidat ima interesovanja i/ili iskustva u toj oblasti.
U nastavku mejla prosleđujemo vam link ka prijavi i oglas u orginalnom
formatu.
Link za prijavu kandidata:
Oglas:
________________________________________
Scientist I – Transgenic mouse line development – Genetic Tools
Department: Molecular Genetics
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Scientist I – Transgenic mouse line development
The mission of the Allen Institute is to unlock the complexities of
bioscience and advance our knowledge to improve human health. Using an
open science, multi-scale, team-oriented approach, the Allen Institute
focuses on accelerating foundational research, developing standards and
models, and cultivating new ideas to make a broad, transformational
impact on science.
The mission of the Allen Institute for Brain Science is to accelerate
the understanding of how the human brain works in health and disease.
Using a big science approach, we generate useful public resources, drive
technological and analytical advances, and discover fundamental brain
properties through integration of experiments, modeling and theory.
The Genetic Tools team (led by Tanya Daigle) within the Molecular
Genetics department (led by Bosiljka Tasic) at the Allen Institute for
Brain Science generates high-precision genetic tools to effectively
target, monitor, and manipulate distinct cell types within the mouse
brain. These genetic tools enable the analysis of the structure and
function of neural circuits within the brain and represent foundational
resources for the neuroscience community.
We aim to generate transgenic mouse driver lines to target a diversity
of neocortical and thalamic cell types and to develop reporter lines in
parallel for functional experiments. We have built many successful
transgene expression platforms (most recently Daigle and Madisen et al.,
Cell 2018) and have demonstrated these tools can be combined with one
another and enhancer-based AAVs (Graybuck and Daigle, et al., Neuron
2021) to target a diversity of cell types. We seek an exceptional
experimentalist to further these platforms and develop intersectional
genetic approaches for cell type targeting. The candidate scientist
should have a strong background in molecular and cellular neuroscience
and mouse genetics and be able to lead efforts to build and validate
transgenic mouse lines. They will design and build targeting vectors,
manage production activities, systematically characterize the lines, and
help manage our transgenic portfolio. This individual should be highly
efficient, thrive in a fast-paced, team environment, and be excited
about developing leading-edge genetic tools for brain cell types. This
work is currently supported by two BRAIN grants, one awarded to Hongkui
Zeng and the other awarded to Karel Svoboda, as well as support from the
Allen Institute for Neural Dynamics.
The Allen Institute believes that team science significantly benefits
from the participation of diverse voices, experiences, and backgrounds.
High-quality science can only be produced when it includes different
perspectives. We are committed to increasing diversity across every team
and encourage people from all backgrounds to apply for this role.
Note: Reasonable accommodations may be made to enable individuals with
disabilities to perform the essential functions. This description
reflects management’s assignment of essential functions; it does not
proscribe or restrict the tasks that may be assigned.
Essential Functions
• Utilize existing in-house analysis tools to explore single-cell
genomics data (scRNA-seq) and identify marker genes for brain regions
and cell types
• Design and construct complex targeting vectors using standard
molecular cloning techniques
• Work with external cores to produce transgenic mice and oversee
breeding of internal mouse colonies
• Evaluate transgene expression in the brain using biochemical
techniques, fluorescence microscopy, immunohistochemistry (IHC), and
multiplex fluorescence in situ hybridization (mFISH)
• Manage characterization efforts conducted by our core teams and
coordinate activities with key stakeholders
• Evaluate new tools, components, and targeting strategies for
implementation in transgenic mouse line production
• Communicate results rapidly and with a high level of transparency to
internal teams and collaborators
• Manage deposition of tools and data to public repositories
• Lead publications, contribute to grant applications, and preparation
of intellectual property (IP)-related materials
Required Education and Experience
• Ph.D. in Neurobiology, Genetics, Biochemistry or related fields; or
equivalent combination of degree and experience
• Experience with molecular cloning techniques and with DNA construction
software (Geneious or Vector NTI)
• Experience with MS office programs and other related software (Adobe
products and software for basic image analysis)
• Ability to work at the bench >80% of the time
Preferred Education and Experience
• Deep experience with molecular cloning techniques and targeting vector
generation
• Experience generating mouse lines
• Experience with complex breeding strategies and characterizing mouse
models
• Experience characterizing gene expression in the mouse brain
• Proficient in mouse brain tissue sectioning, staining (IHC),
fluorescence imaging (epi and confocal), protein and image analysis
• Outstanding oral and written communication skills
• Experience working in milestone-driven environment with aggressive
timelines
• Highly self-motivated, experienced research scientist with a positive
attitude and ability to work independently to solve problems
• Experience designing and leading research projects
• Strong publication record
Work Environment
• Will enter laboratory environment, including potential exposure to
lasers, biohazards
Physical Demands
• Fine motor movements in fingers/hands to operate computers and other
office equipment; repetitive motion with lab equipment
Position Type/Expected Hours of Work
• This role is currently working onsite and remotely when possible, due
to COVID-19 and our focus on employee safety. We continue to evaluate
the safest options for our employees.
Additional Comments
• **Please note, this opportunity offers relocation assistance**
• **Please note, this opportunity offers work visa sponsorship**
It is the policy of the Allen Institute to provide equal employment
opportunity (EEO) to all persons regardless of age, color, national
origin, citizenship status, physical or mental disability, race,
religion, creed, gender, sex, sexual orientation, gender identity and/or
expression, genetic information, marital status, status with regard to
public assistance, veteran status, or any other characteristic protected
by federal, state or local law. In addition, the Allen Institute will
provide reasonable accommodations for qualified individuals with
disabilities.
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